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KMID : 0624620110440030199
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BMB Reports
2011 Volume.44 No. 3 p.199 ~ p.204
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EphrinB1 interacts with the transcriptional co-repressor Groucho/xTLE4
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Kamata Teddy
Bong Yong-Sik
Mood Kathleen
Nishanian Tagvor G.
Lee Hyun-Shik
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Abstract
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Ephrin signaling is involved in various morphogenetic events, such as axon guidance, hindbrain segmentation, and angiogenesis. We conducted a yeast two-hybrid screen using the intracellular domain (ICD) of EphrinB1 to gain biochemical insightinto the function of the EphrinB1 ICD. We identified the transcriptional co-repressor xTLE1/Groucho as an EphrinB1 interacting protein. Whole-mount in situ hybridization of Xenopus embryos confirmed the co-localization of EphrinB1 and a Xenopus counterpart to TLE1, xTLE4, during various stages of development. The EphrinB1/xTLE4 interaction was confirmed by co-immunoprecipitation experiments. Further characterization of the interaction revealed that the carboxy-terminal PDZ binding motif of EphrinB1 and the SP domain of xTLE4 are required for binding. Additionally, phosphorylation of EphrinB1 by a constitutively activated fibroblast growth factor receptor resulted in loss of the interaction, suggesting that the interaction is modulated by tyrosine phosphorylation of the EphrinB1 ICD.
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KEYWORD
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Eph, EphrinB1, Groucho, Xenopus, xTLE4
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